AAV 滴定定量斑点印迹法及其在腺相关病毒组装活化蛋白功能评价中的应用
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14:49 min
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June 12, 2018
DOI:
成績單
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Summary
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本手稿详细介绍了一种直接斑点印迹法定量的腺相关病毒 (AAV) 的效价及其应用研究组装活化蛋白 (AAPs) 的作用, 一种新型的非结构化病毒蛋白在所有 AAV 发现血清型, 促进衣壳的组装从同源和异源 AAV 血清型。
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Cite this Article
Powers, J. M., Chang, X. L., Song, Z., Nakai, H. A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins. J. Vis. Exp. (136), e56766, doi:10.3791/56766 (2018).
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