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Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter Assay Platforms

Article DOI: 10.3791/56795-v • 06:40 min • January 25th, 2018
January 25th, 2018 •
*1, *1, *1, 1, 1, 1, 1
1Laboratory of Molecular Neurobiology, Institute of Cellular and Organismic Biology, Academia Sinica
* These authors contributed equally

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We have successfully generated two substrate-specific γ-secretase assays. Both cell-based assays presented here are designed to quantify γ-secretase enzymatic activities via the output of firefly luciferase reporters.

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Quantitative Measurement γ-secretase Amyloid Precursor Protein Notch Cleavage Cell-based Luciferase Reporter Assay Platforms Putative γ-secretase Modulators Alzheimer's Disease Assay System γ-secretase Modulators Anti-AD Drugs Substrate Selectivity Neuroblastoma Cell Culture Media 96 Microplate NG Cells CG Cells Hemocytometer
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