CRISPR/Cas9-mediated Targeted Integration <em>In Vivo</em> Using a Homology-mediated End Joining-based Strategy

Xuan Yao; Xing Wang; Junlai Liu; Linyu Shi; Pengyu Huang; Hui Yang

doi:10.3791/56844

Journal

/

遗传学

/

CRISPR/Cas9-mediated 目标集成在体内使用基于同源介导的端接连接策略

CRISPR/Cas9-mediated Targeted Integration <em>In Vivo</em> Using a Homology-mediated End Joining-based Strategy

JoVE 杂志
遗传学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志遗传学

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

CRISPR/Cas9-mediated 目标集成在体内使用基于同源介导的端接连接策略

Please note that all translations are automatically generated. Click here for the English version.

14,761 Views

•

08:22 min

•

March 12, 2018

DOI:

10.3791/56844-v

DOI:

10.3791/56844-v

•

08:22 min

March 12, 2018

•

6 Views

Xuan Yao^1,2, Xing Wang^1,2, Junlai Liu^2,3,4, Linyu Shi¹, Pengyu Huang³, Hui Yang¹

¹Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, ²College of Life Sciences,University of Chinese Academy of Sciences, ³School of Life Science and Technology,Shanghai Tech University, ⁴Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences

成績單

Summary

聚簇定期 interspaced 短回文重复/CRISPR 相关蛋白 9 (CRISPR/Cas9) 系统为基因工程提供了一个有前途的工具, 并开辟了转基因目标整合的可能性。我们描述了一种基于同源介导的端接 (HMEJ) 策略, 用于有效的 DNA 靶向集成体内和使用 CRISPR/Cas9 的靶向基因治疗。