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Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies
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JoVE 杂志 免疫与感染
Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

DOI:
10.3791/56949-v

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06:56 min

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March 02, 2018

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, , ,
1Department of Pathology, Feinberg School of Medicine,Northwestern University

Chapters

  • 00:05Title
  • 00:55Murine Bone Marrow Polymorphonuclear (PMN) Cell Separation
  • 02:37Microparticle (MP) Isolation from Bone Marrow-derived Activated Murine PMNs
  • 03:45In Vivo PMN-derived Microparticle (MP) Wound Healing Assay
  • 04:38Results: Representative Effects of Isolated PMN-MPs in Epithelial Wound Healing
  • 05:36Conclusion

Summary

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New protocols are described here to isolate and characterize microparticles derived from human and mouse neutrophils. These protocols utilize ultracentrifugation, flow cytometry, and immunoblotting techniques to analyze microparticle content, and they can be used to study the role of microparticles derived from various cell types in cellular function.

Tags

Neutrophil-derived MicroparticlesCell-cell CommunicationDensity Gradient SeparationPolymorphonuclear CellsPMNMononuclear CellsCell-free SupernatantsUltra-centrifugationFunctional Studies

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