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Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Article DOI: 10.3791/57087-v • 14:23 min • March 6th, 2018
March 6th, 2018 •
1, 2, 2, 1
1Cell Polarity, Migration and Cancer Unit, UMR 3691, CNRS, Institut Pasteur, 2UTechS Photonic BioImaging (Imagopole) Citech, Institut Pasteur

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The overall goal of this methodology is to give the optimal experimental conditions from sample preparation to image acquisition and reconstruction in order to perform 2D dual color dSTORM images of microtubules and intermediate filaments in fixed cells

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2-dimensional Direct Stochastic Optical Reconstruction Microscopy Sample Preparation Image Acquisition Reconstruction 2D Dual-colored D-STORM Imaging Microtubules Intermediate Filaments Fixed Cells Cytoskeletal Networks Fluorescence Microscopy Sample Preparation Control Artifacts U373 Cells Extraction Fixation Solution 4%PFA Cytoskeleton Buffer Incubator PBS
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