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Immunology and Infection

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Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies

Article DOI: 10.3791/57100-v • 12:32 min • March 13th, 2018
March 13th, 2018 •
1, 1, 1, 1
1Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth

章节

总结概括
Automatic Translation

We describe here a method to isolate and purify dendritic cells from different anatomical compartments in the human female reproductive tract for the evaluation of their phenotypical and functional characteristics. This method can be adapted to isolate other immune cells or dendritic cells from other mucosal tissues.

Tags

Isolate Dendritic Cells Human Female Reproductive Tract Phenotypical Studies Functional Studies Anatomical Compartments Tissue-resident Dendritic Cells Female Genital Tract Tissue Digestion Protocol Surface Markers Isolation Technique Immune Cells Other Tissues Fiona Barr Jared Fortier Technicians Modified HBSS Digestion Enzyme Cocktail
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