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Bioengineering
早期雏鸡胚胎形态发生中物理力的作用探讨
早期雏鸡胚胎形态发生中物理力的作用探讨
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis

早期雏鸡胚胎形态发生中物理力的作用探讨

Full Text
7,727 Views
06:33 min
June 5, 2018

DOI: 10.3791/57150-v

Yan Li*1, Hannah Grover*1, Eric Dai2, Kevin Yang1, Zi Chen1

1Thayer School of Engineering,Dartmouth College, 2Department of Bioengineering,University of Pennsylvania

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Please note that some of the translations on this page are AI generated. Click here for the English version.

在这里, 我们提出了一个协议, 介绍了一套新的前蛋实验和物理建模方法来研究早期鸡胚脑扭转的形态发生机制。

该实验方案的总体目标是通过卵外实验研究机械力在早期胚胎大脑发育中的作用。这种方法可以帮助回答发育生物学和生物力学中的关键问题,例如机械线索在形态发生中的作用,形态发生是生物形式的产生。该技术的主要优点是它能够在组织和生物体水平上表征机械力在形态发生中的作用。

要开始此过程,请使用含有 70% 乙醇的精致湿巾清洁受精的不含特定病原体的 White Leghorn 鸡蛋。然后,将鸡蛋纵向排列在支架上。之后,将鸡蛋孵化器设置在 37.5 摄氏度的目标温度,并将湿度保持在 48% 至 55%,通过向孵化器中加入适量的水来控制湿度。

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