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JoVE Journal Bioengineering
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
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Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Article DOI: 10.3791/57166-v • 06:47 min • May 4th, 2018
May 4th, 2018 •
1,2, 3, 1,4,5,6, 1,7,8
1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University

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Automatic Translation

We present a protocol to investigate the mRNA expression biomarkers of periosteum-derived cells (PDCs) induced by vitamin C (vitamin C) and 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. In addition, we evaluate the ability of PDCs to differentiate into osteocytes, chondrocytes, and adipocytes.

Tags

Mesenchymal Stem Cells Alveolar Periosteum Vitamin D Osteogenic Activity Dental Tissue Bone Formation Laboratory Staff Hsin-Wen Chi Periosteal Separator Tissue Slices DPBS Antibiotics Culturing Medium Petri Dishes
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