Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Here, we present two protocols for the measurement of mitochondrial Ca²⁺ influx in isolated mitochondria and cultured cells. For isolated mitochondria, we detail a plate reader-based Ca²⁺ import assay using the Ca²⁺ sensitive dye calcium green-5N. For cultured cells, we describe a confocal microscopy method using the Ca²⁺ dye Rhod-2/AM.