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Immunology and Infection

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JoVE Journal Immunology and Infection
Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
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Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

Article DOI: 10.3791/57283-v • 08:13 min • May 7th, 2018
May 7th, 2018 •
*1, *1, *1,2, 1
1Theodor-Kocher Institute, University of Bern, 2Center for Infectious Diseases, Integrative Virology, University Clinic of Heidelberg
* These authors contributed equally

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总结概括
Automatic Translation

We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.

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Murine Submandibular Salivary Gland Upright Intravital Microscopy Surgical Procedure Immunology Research Model Organ Advantage Fur Removal Hair Removal Cream Wet Tissues Stage Preparation Heating Ring Removable Coverslip Holder Ear Bar Fixed Coverslip Holders Gauze Bedding Coverglass Holders Positioning The Mouse Surgical Tape
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