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DOI: 10.3791/57290-v
Renee Wei-Yan Chow*1,2,3,4, Paola Lamperti*1,2,3,4, Emily Steed1,2,3,4, Francesco Boselli1,2,3,4, Julien Vermot1,2,3,4
1Institut de Génétique et de Biologie Moléculaire et Cellulaire, 2UMR7104,Centre National de la Recherche Scientifique, 3U964,Institut National de la Santé et de la Recherche Médicale, 4Université de Strasbourg
Please note that some of the translations on this page are AI generated. Click here for the English version.
该协议描述了在活斑马鱼胚胎的心内膜细胞中 photoconversion 枫荧光蛋白的方法, 能够在房室管和房室心脏瓣膜发育过程中跟踪心内膜细胞。.
该程序的总体目标是在房室管和心脏瓣膜形成过程中跟踪心内膜细胞。这种方法可以帮助解决心脏发育领域的关键问题,例如了解心内膜细胞在不同类型突变体中的行为。由于心脏的快速跳动,在 AVC 和瓣膜形成期间追踪细胞具有挑战性。
这使得通过传统的延时显微镜跟踪细胞变得困难。这种技术的主要优点是它绕过了其中一些困难。演示该程序的是我实验室的博士后 Renee。
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