利用Cas9核糖核蛋白在多种细胞和生物体中实现增强的基因组编辑
37K 次观看
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被引用 45 次
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09:51 分钟
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2018年5月25日
DOI :10.3791/57350-v
2018年5月25日
•37K 次观看
Behnom Farboud*1,2 , Erin Jarvis*1 , Theodore L. Roth*3,4,5,6 , Jiyung Shin*1,3 , Jacob E. Corn1,3 , Alexander Marson3,5,6,7,8,9 , Barbara J. Meyer1,2 , Nipam H. Patel1,10 , Megan L. Hochstrasser3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally
利用预先组装的Cas9核糖核蛋白复合物（RNP）是一种实现精确、高效基因组编辑的有力方法。本文重点介绍该方法在多种细胞和生物体中的应用，包括原代人源细胞以及经典和新兴的模式生物。
Chapters in this video
0:04
Title
1:09
RNP Preparation for Primary Cell Editing, Hematopoietic Stem/Progenitor Cell (HSPC) Preparation, and RNP Electroporation
2:46
C. elegans Gonad Microinjection with RNPs and Post-injection Care
5:17
P. hawaiensis Embryo Microinjection with RNPs and Post-injection Care
8:05
Results: Evaluation of Genome Editing Outcomes
9:28
Conclusion
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