用最小组织量测定果蝇透纤维中线粒体耗氧量
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April 07, 2018
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本文介绍了果蝇透胸部高分辨率 respirometry 测量耗氧量的方法。与经典的线粒体分离技术相比, 这种技术需要最少的组织量, 获得的结果与生理相关。
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Cite this Article
Simard, C. J., Pelletier, G., Boudreau, L. H., Hebert-Chatelain, E., Pichaud, N. Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue. J. Vis. Exp. (134), e57376, doi:10.3791/57376 (2018).
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