Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

Michal Adamski; Gianluca Fontana; Joshua R. Gershlak; Glenn R. Gaudette; Hau D. Le; William L. Murphy

doi:10.3791/57586

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

组织工程应用中植物组织去细胞的两种方法

Full Text

15,446 Views

05:20 min

May 31, 2018

DOI: 10.3791/57586-v

Michal Adamski¹, Gianluca Fontana², Joshua R. Gershlak⁴, Glenn R. Gaudette⁴, Hau D. Le¹, William L. Murphy^2,3

¹Department of Surgery,University of Wisconsin-Madison, ²Department of Orthopedics and Rehabilitation,University of Wisconsin School of Medicine and Public Health, ³Department of Biomedical Engineering,University of Wisconsin College of Engineering, ⁴Department of Biomedical Engineering,Worcester Polytechnic Institute

Please note that some of the translations on this page are AI generated. Click here for the English version.

在这里, 我们介绍, 并对比两个协议用于 decellularize 植物组织: 洗涤剂为基础的方法和无洗涤剂的方法。这两种方法都留下了植物组织的细胞外基质, 然后可以用作组织工程应用的支架。

这些方法可以帮助解决生物材料领域的关键问题，例如开发具有出色流体传输能力、复杂超结构和可扩展制造的支架。这些技术的主要优点是它们可以应用于大多数植物的干细胞组织，而无需专门的设备。首先，收集新鲜或冷冻的 F.hispida 叶样品。

将剩余的新鲜样品储存在零下 20 摄氏度以备将来使用。让叶子在 20 至 25 摄氏度的去离子水中保持浸泡。使用干净的活检打孔器将叶子切成直径为 8 毫米的圆盘。

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

生物工程问题 135 瓣膜支架植物组织叶子茎 perfusable 支架去细胞