Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Hsi-Ju Wei; John J. Letterio; Tej K. Pareek

doi:10.3791/57637

JoVE Journal
Medicine

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JoVE Journal Medicine

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

小鼠致耐受性树突状细胞的发育及功能表征

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09:51 min

May 18, 2018

DOI: 10.3791/57637-v

Hsi-Ju Wei¹, John J. Letterio^2,3, Tej K. Pareek^2,3

¹Department of Biochemistry, School of Medicine,Case Western Reserve University, ²Department of Pediatrics, Division of Pediatric Hematology/Oncology,Case Western Reserve University, ³Angie Fowler Cancer Institute, Rainbow Babies & Children's Hospital,University Hospitals, Cleveland

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在这里, 我们提出了一个协议, 开发和表征致耐受性树突状细胞 (TolDCs), 并评估其免疫治疗效用。

该方案的总体目标是开发和表征小鼠致耐受性树突状细胞，以评估它们在治疗自身免疫性疾病（如多发性硬化症）中的免疫治疗效用。该方法通过提供一种标准化方法来开发和表征致耐受性树突状细胞，可以帮助回答致耐受性树突状细胞治疗领域的关键问题。该技术的主要优点是可用于成功的致耐受性树突状细胞发育和功能性致耐受性树突状细胞功效测试。

这种技术的意义是巨大的。它们延伸到自身免疫性疾病的细胞免疫疗法的开发，因为致耐受性树突状细胞可以重置异常免疫反应，同时保持受内在免疫调节机制的约束。根据标准方案，从 8 至 10 周龄的 C57BL/6 小鼠收获后腿骨后，使用手术刀片和镊子解剖尽可能多的组织，并将干净的胫骨和股骨放入含有 70% 乙醇的 6 厘米培养皿中。

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