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JoVE Journal
Environment
流式细胞仪对水生生物膜的表征
流式细胞仪对水生生物膜的表征
JoVE Journal
Environment
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JoVE Journal Environment
Characterization of Aquatic Biofilms with Flow Cytometry

流式细胞仪对水生生物膜的表征

Full Text
9,665 Views
08:30 min
June 6, 2018

DOI: 10.3791/57655-v

Linn Sgier1, Stephanie N. Merbt1, Ahmed Tlili1, Alexandra Kroll1, Anze Zupanic1

1Department of Environmental Toxicology,Eawag - Swiss Federal Institute for Aquatic Science and Technology

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Please note that some of the translations on this page are AI generated. Click here for the English version.

流式细胞术与视觉聚类结合, 为研究水生生物膜提供了一种简便、快速的方法。它可用于生物膜的表征, 检测生物膜群落结构的变化, 以及检测埋在生物膜中的非生物微粒。

这种方法可以帮助回答环境科学领域的关键问题,例如化学污染或气候变化对水生生态系统的影响。这种技术的主要优点是所有单独的步骤都简单快速。虽然该程序的重点是表征自养生物膜,但它也可用于表征异养生物膜和检测颗粒污染物,例如微塑料。

首先,选择生物膜生长的水生采样点,这些通常是溪流的浅水部分,具有缓慢到中等的水流和石质河床。如果该部位没有足够的表面来附着生物膜,您可以使用可以生长生物膜的人造基质。使用便携式仪器测量采样现场的环境条件,以测量待采样生物膜表面正上方的电导率、pH 值、水温和光强度。

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