The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen <em>Magnaporthe Grisea</em>

Mengyu Zhang; Xuan Sun; Lie Cui; Yue Yin; Xinyu Zhao; Song Pan; Weixiang Wang

doi:10.3791/57675

JoVE Journal
Environment

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Environment

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

植物感染试验: 以植物病原菌为载体的喷雾和伤口介导接种

Full Text

13,406 Views

07:14 min

August 4, 2018

DOI: 10.3791/57675-v

Mengyu Zhang*¹, Xuan Sun*¹, Lie Cui¹, Yue Yin¹, Xinyu Zhao¹, Song Pan², Weixiang Wang¹

¹Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education,Beijing University of Agriculture, ²College of Plant Protection, State Key Laboratory of Agrobiotechnology, Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management,China Agricultural University

Please note that some of the translations on this page are AI generated. Click here for the English version.

在这里, 我们提出了一个测试植物致病力的植物病原菌稻瘟病的协议.这份报告将有助于大规模筛选真菌分离株的致病型, 作为了解植物在分子育种过程中耐药性机制的良好出发点。

该视频可以帮助指导植物感染领域的关键方法，例如用植物病原体 Magnaporthe grisea 进行游离和想要介导的接种。该技术的主要优点是喷洒、伤害方案有助于快速、准确和大规模筛选真菌分离物的病理类型。这项技术的意义与对抗植物病原体的生理学研究有关，因为它对于预防和控制大规模植物病害非常重要。

该方法可深入了解真菌的保守致病机制。它也可以应用于水稻或大麦等主要农业植物品种。通常，刚接触这种方法的人会很挣扎，因为很难用无菌棉签轻轻刮擦分生孢子。

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

环境科学问题 138 植物毒力稻瘟病菌传染稻瘟病大麦接种