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Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Article DOI: 10.3791/57723-v • 10:08 min • June 8th, 2018
June 8th, 2018 •
1, 2, 2, 2
1Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, 2Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health

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Challenging young neurons in new brain regions can reveal important insights into how the environment sculpts neuronal fate and maturation. This protocol describes a procedure to harvest interneuron precursors from specific brain regions and transplant them either homotopically or heterotopically into the brain of postnatal pups.

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Homochronic Transplantation Interneuron Precursors Postnatal Mouse Brains Environmental Changes Developmental Neuroscience Genetic Programs Neural Cells Fate And Maturation Adoptive Transfer Cell Harvesting Grafting Steps Transplant Experiments Razor Blades Striatal Slices Petri Dish Sucrose Artificial Cerebrospinal Fluid (sACSF) Dissecting Microscope Forceps
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