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Immunology and Infection

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Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

Article DOI: 10.3791/57767-v • 08:03 min • July 12th, 2018
July 12th, 2018 •
1, 1, 1, 1
1Department of Microbiology, Immunology, and Biochemistry, Wayne State University School of Medicine

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Zebrafish are a natural Vibrio cholerae host and can be used to recapitulate and study the entire infectious cycle from colonization to transmission. Here, we demonstrate how to assess V. cholerae colonization levels and quantify diarrhea in zebrafish.

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Quantifying Vibrio Cholerae Colonization Diarrhea Adult Zebrafish Model Technique Vibrio Cholerae Field Quiet Coast Intestinal Microbiota Niche Advantage Fish Host Life Cycle Procedure Dhrubajyoti Nag Paul Breen V.cholerae Cultures Inoculate Zebrafish Immersion Beaker Infection Water Infection Concentration Perforated Lid Glass Front Incubator Transitory Exposure Fish Net Infected Water Bleach Disposal Sterile Infection Water
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