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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Article DOI: 10.3791/57971-v • 09:34 min • July 27th, 2018
July 27th, 2018 •
*1, *1, 1
1The Autophagy Team, Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo
* These authors contributed equally

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Here a simple and well-validated protocol for measuring bulk autophagic sequestration activity in mammalian cells is described. The method is based on quantifying the proportion of lactate dehydrogenase (LDH) in sedimentable cell fractions compared to total cellular LDH levels.

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Lactate Dehydrogenase Sequestration Assay Autophagic Sequestration Activity Mammalian Cells Bulk Autophagy Cargo Sequestration Endogenous Cargo Overexpression Cargo Probes Paula Szalai Morten Luhr Culture Adherent Cells Tissue Culture Flasks PBS Trypsin EDTA Culture Medium FBS Sterile Tube Trypan Blue Counting Chamber Slide
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