<em>Saccharomyces cerevisiae</em> Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Tiago Baptista; Didier Devys

doi:10.3791/57982

Journal

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遗传学

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酿酒酵母4-硫尿嘧啶代谢标记及新合成 mRNA 作为 RNA 聚合酶 II. 活性的一种代理

<em>Saccharomyces cerevisiae</em> Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

酿酒酵母4-硫尿嘧啶代谢标记及新合成 mRNA 作为 RNA 聚合酶 II. 活性的一种代理

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9,089 Views

•

09:21 min

•

October 22, 2018

DOI:

10.3791/57982-v

DOI:

10.3791/57982-v

•

09:21 min

October 22, 2018

•

9083 Views

Tiago Baptista^1,2,3,4, Didier Devys^1,2,3,4

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France ²Centre National de la Recherche Scientifique, Illkirch, France ³Institut National de la Santé et de la Recherche Médicale, Illkirch, France ⁴Université de Strasbourg

Summary

这里描述的协议是基于全基因组的定量的新合成的 mRNA 纯化的酵母细胞标记为 4-硫尿嘧啶。这种方法允许测量 mrna 合成与 mrna 衰变分离, 从而提供了 RNA 聚合酶 II 转录的精确测量。

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酿酒酵母4-硫尿嘧啶代谢标记及新合成 mRNA 作为 RNA 聚合酶 II. 活性的一种代理

Summary

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