Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Zhi-Xiong Zhou; Jessica S. Williams; Thomas A. Kunkel

doi:10.3791/58020

Journal

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生物学

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研究核苷酸合并: 酵母基因组 Ribonucleotides 的链特异检测及核苷酸诱变的检测

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

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Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

研究核苷酸合并: 酵母基因组 Ribonucleotides 的链特异检测及核苷酸诱变的检测

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7,686 Views

•

09:04 min

•

July 26, 2018

DOI:

10.3791/58020-v

DOI:

10.3791/58020-v

•

09:04 min

July 26, 2018

•

5 Views

Zhi-Xiong Zhou*¹, Jessica S. Williams*¹, Thomas A. Kunkel¹

¹Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, NIH, DHHS

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Summary

Ribonucleotides 是在核 DNA 复制过程中纳入基因组的最丰富的非规范核苷酸之一。如果没有正确去除, ribonucleotides 会导致 DNA 损伤和突变。在这里, 我们提出了两个实验方法, 用于评估核苷酸的丰度纳入 DNA 及其诱变效应。

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Ribonucleotide Incorporation Strand-specific Detection Yeast Genome Ribonucleotide-induced Mutagenesis Alkaline Gel Electrophoresis Southern Blotting Genomic DNA Potassium Hydroxide Alkaline DNA Loading Buffer Agarose Gel Sodium Hydroxide EDTA DNA Size Marker Neutralization Buffer SYBR Gold Stain UV Transilluminator Alkaline Transfer Buffer Nylon Membrane Capillary Action

研究核苷酸合并: 酵母基因组 Ribonucleotides 的链特异检测及核苷酸诱变的检测

成績單

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Summary

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