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A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

Article DOI: 10.3791/58208-v • 05:36 min • September 6th, 2018
September 6th, 2018 •
1, 1
1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)

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A long-term culture model of bovine granulosa cells under serum-free conditions is described. This model allows researchers to study the effects of diverse factors and conditions as different plating densities on the characteristics of estrogen-producing bovine granulosa cells.

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Tissue Culture Model Estrogen-producing Primary Bovine Granulosa Cells Estradiol Thyroid Factors Cryopreservation Technique Cell Isolation Procedure Wash Bovine Ovaries Antibiotic Supplemented 1X PBS Puncture Follicles Follicular Fluid Granulosa Cells 18 Gauge Needle 50 Milliliter Centrifuge Tube Freshly Isolated GCs RNA Analysis DNA Analysis Protein Analysis
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