用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板
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09:36 min
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November 06, 2018
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节点和脊索板是小鼠胚胎发育过程中的瞬态信号组织者, 可以使用多种技术进行可视化。在这里, 我们详细地描述了如何执行两种技术来研究它们的结构和形态发生: 1) 扫描电子显微镜 (sem);和 2) 全安装免疫荧光 (wmif)。
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Cite this Article
Xiao, C., Nitsche, F., Bazzi, H. Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence. J. Vis. Exp. (141), e58321, doi:10.3791/58321 (2018).
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