Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Michael Surace; Karma DaCosta; Anna Huntley; Weiguang Zhao; Christopher Bagnall; Charles Brown; Chichung Wang; Kristin Roman; Jennifer Cann; Arthur Lewis; Keith Steele; Marlon Rebelatto; Edwin R. Parra; Clifford C. Hoyt; Jaime Rodriguez-Canales

doi:10.3791/58390

JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Cancer Research

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

福尔马林固定癌组织标本免疫肿瘤学自动复合免疫荧光小组研究

Full Text

21,566 Views

10:49 min

January 21, 2019

DOI: 10.3791/58390-v

Michael Surace¹, Karma DaCosta¹, Anna Huntley¹, Weiguang Zhao¹, Christopher Bagnall², Charles Brown¹, Chichung Wang³, Kristin Roman³, Jennifer Cann¹, Arthur Lewis², Keith Steele¹, Marlon Rebelatto¹, Edwin R. Parra⁴, Clifford C. Hoyt³, Jaime Rodriguez-Canales¹

¹Laboratory of Pathology,MedImmune, ²Laboratory of Pathology,MedImmune, ³Akoya Biosciences Inc., ⁴Department of Translational Molecular Pathology,University of Texas - MD Anderson Cancer Center

Please note that some of the translations on this page are AI generated. Click here for the English version.

对6个标记多重免疫荧光面板的详细协议进行了优化和执行, 使用自动着色器实现更一致的结果和更短的手术时间。这种方法可以直接由任何实验室用于免疫肿瘤学研究。

该协议意义重大，因为多路复用免疫荧光协议的发展优化和转移是复杂和具有挑战性的。对于许多实验室来说，优化协议是无价的。多光谱免疫荧光使我们能够评估肿瘤内和周围多种细胞类型的位置和标识。

这是免疫肿瘤学研究中信息的有力组合。多路复用免疫荧光最吸引人的用途是免疫肿瘤学。但是，在所有自身免疫和炎症疗法中，都可以利用对 setu 中的多种免疫细胞类型进行测量。

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

癌症研究第143期多重免疫荧光多光谱成像免疫组织化学免疫肿瘤学免疫分析肺癌