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Immunology and Infection

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Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

Article DOI: 10.3791/58453-v • 05:39 min • October 30th, 2018
October 30th, 2018 •
*1, *1, 1,2, 1,3,4,5
1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2MedEngine Oy, 3Department of Pediatrics, Tampere University Hospital, 4Department of Children and Adolescents, Oulu University Hospital, 5PEDEGO Research Unit, Medical Research Center, University of Oulu
* These authors contributed equally

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Here we describe a protocol for the immunization of the adult zebrafish (Danio rerio) with a DNA-based vaccine and demonstrate the validation of a successful vaccination event. This method is suitable for the preclinical screening of vaccine candidates in various infection models.

Tags

Immunization Adult Zebrafish Preclinical Screening DNA-based Vaccines Tuberculosis Infectious Diseases Cost-effective Procedure Novel Vaccine Candidates Hannaleena Piippo Laboratory Technician Glass Capillary V-groove Clamping Knob Filament Polar Bar Safety Glass Pull Button Needles Reusable Adhesive Petri Dish Injection Mix Plasmid Per Dose Master Mix Capillary Needle Tape Holder Droplets Of Plasmid Doses Laboratory Film Loading Tip, Micro Manipulator Light Source
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