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Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
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Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Article DOI: 10.3791/58613-v • 08:58 min • June 19th, 2019
June 19th, 2019 •
*1, *2, 2,3
1Department of Molecular Neuroscience and Integrative Physiology, Faculty of Medicine, Kanazawa University, 2International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 3Faculty of Medicine, University of Tsukuba
* These authors contributed equally

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Here, we describe methods of optogenetic manipulation of particular types of neurons during monitoring of sleep/wakefulness states in mice, presenting our recent work on the bed nucleus of the stria terminalis as an example.

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Optogenetic Manipulation Neural Circuits Sleep/wakefulness States Mice Monitoring Less Invasive Manipulation High Temporal Resolution Surface EEG Causal Relationship Shota Kodani Procedure Toe Pinch Ointment Ear Bars Nose Pin Stereotactic Apparatus Anesthetized Mouse Absorbent Pad Mid-sagittal Incision Scalp Sutura Sagittalis Sutura Coronalis Sutura Lambdoidea Positioning Gap Serafin Clamps Skin Open Disinfect Exposed Surface Of The Skull Cranial Sutures Adeno-associated Virus Vector Ethanol Sterilized 10 Milliliter Syringe Mineral Oil
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