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Bioengineering
基于多步法链位移光刻的基因功能表面固定化研究
基于多步法链位移光刻的基因功能表面固定化研究
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

基于多步法链位移光刻的基因功能表面固定化研究

Full Text
7,968 Views
11:05 min
October 25, 2018

DOI: 10.3791/58634-v

Günther Pardatscher*1, Matthaeus Schwarz-Schilling*1, Sandra Sagredo1, Friedrich C. Simmel1

1Physics Department,Technical University of Munich

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Please note that some of the translations on this page are AI generated. Click here for the English version.

我们描述了一种简单的光刻程序, 用于固定在表面上的基因长度 DNA 分子, 可用于在生物芯片上执行无细胞基因表达实验。

DNA生物芯片是巴斯夫集团最初开发的自合成生物学的有吸引力的研究平台,它允许在较长时期内进行体外基因回路的运行。通过这个协议,我们希望使这项技术提供给更广泛的研究人员。我们的方法,即贝福光刻,代表了基于DNA链位移反应的DNA固定策略。

该技术的优势在于其多步平版学功能、简单的可重复性以及所有组件的商业可用性。与其他生物芯片解决方案类似,贝福可以与不同的技术结合使用。例如,功能基因刷可以组装在微室中进行蛋白质表达。

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