小鼠棕色脂肪组织的染色质免疫沉淀
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07:50 min
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November 21, 2018
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Summary
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在这里, 我们描述了有效染色质免疫沉淀 (chip) 的协议, 然后是从小鼠身上分离出的棕色脂肪组织 (bat) 的高通量 dna 测序 (chip-seq)。该方案既适用于组蛋白修饰的映射, 也适用于研究感兴趣的非组蛋白在体内的全基因组定位。
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Cite this Article
Cardamone, M. D., Orofino, J., Labadorf, A., Perissi, V. Chromatin Immunoprecipitation of Murine Brown Adipose Tissue. J. Vis. Exp. (141), e58682, doi:10.3791/58682 (2018).
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