Using Enhanced Green Fluorescence Protein-expressing <em>Escherichia Coli</em> to Assess Mouse Peritoneal Macrophage Phagocytosis

Yu Zhang&#160;; Guan Wang; Jia Lu; Li-ming Xu; Jun-yu Xiong

doi:10.3791/58751

JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection

Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis

增强型绿色荧光蛋白表达大肠杆菌评价小鼠腹腔巨噬细胞吞噬细胞增多

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12:35 min

January 4, 2019

DOI: 10.3791/58751-v

Yu Zhang *¹, Guan Wang*¹, Jia Lu¹, Li-ming Xu², Jun-yu Xiong¹

¹Anesthesiology Department,The Second Hospital of Dalian Medical University, ²Scientific Research Center,The Second Hospital of Dalian Medical University

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在这里, 我们提出了一个评估小鼠腹腔巨噬细胞吞噬使用增强绿色荧光蛋白表达大肠杆菌的方案.

该协议演示了一种简单和可产生的方法，使用EGFP表达大肠杆菌评估巨噬细胞的噬菌体细胞形成能力。你好，我叫大连医科大学第二医院的俊宇。今天，我们将向您展示一个简单和可视化的方式来评估巨噬细胞病，可以很容易地在两个小时内完成。

该方法在先天免疫功能的研究中得到了广泛的应用。据认为，老年人的先天免疫功能仍然完好无损。因此，今天我们将从老年和年轻的小鼠中分离出内酮巨噬细胞，并了解这两组小鼠的吞噬能力。

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免疫学与感染第142期巨噬细胞吞噬小鼠原代细胞培养细胞衰老增强型绿色荧光蛋白