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JoVE Journal Cancer Research
Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
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Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Article DOI: 10.3791/58758-v • 09:07 min • December 19th, 2018
December 19th, 2018 •
1, 1
1Reproductive Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health (NIH)

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We present a method for analyzing the 4-hydroxy-tamoxifen-dependent estrogen receptor alpha ligand-binding domain dimerization activity using the mammalian two-hybrid assay.

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Ligand-binding Domain Estrogen Receptor Alpha Mammalian Two-hybrid Assay Ligand-dependent Dimerization Activity Selective Estrogen Receptor Modulators Simple Method Plasmids Luciferase Reporter Plasmid Galactose-responsive Transcription Factor GAL4 PG5-Luc Reporter Herpes Simplex Virus Transactivating Segment Protein VP16
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