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Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Article DOI: 10.3791/58952-v • 08:59 min • February 12th, 2019
February 12th, 2019 •
1,2, 1, 1, 1, 1, 1
1Cellular Protein Chemistry, Utrecht University, 2Program in Cellular and Molecular Medicine, Boston Children's Hospital

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Here we describe a protocol for a general pulse-chase method that allows the kinetic analysis of folding, transport, and degradation of proteins to be followed in live cells.

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Protein Folding Protein Transport Protein Degradation Radioactive Pulse Chase 35S-labeled Methionine 35S-labeled Cysteine Protein Biosynthesis Translation Folding And Assembly Trafficking And Degradation Radioactive Workspace Buffers Clear Schedule Microscopy Inspection Wash Buffer Starvation Medium Humidified Incubator Carbon Dioxide Water Bath
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