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An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay

Article DOI: 10.3791/59025-v • 09:14 min • January 26th, 2019
January 26th, 2019 •
1,2, 1, 1, 1, 1, 3, 1,2,4, 1,2
1Murdoch Children's Research Institute, 2Department of Paediatrics, University of Melbourne, 3School of Chemistry and Molecular Bioscience, University of Queensland, 4London School of Hygiene and Tropical Medicine

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This study describes a high throughput, imaging-based micro-neutralization assay to determine the titer of neutralizing antibodies specific for respiratory syncytial virus (RSV). This assay format has been tested on different sample types.

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Improved High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay Accurate Measuring Neutralizing Antibodies Protection Reproducible Inter-assay Variations Reference Antiserum Laboratories Low Cost Imaging-based Subgroup A Subgroup B Sample Types Reuben Tse Research Team MCRI Seed Plates A549 Cells DMEM Media FCS (Fetal Calf Serum) Penicillin-Streptomycin Mixture Flat-bottom Sterile Plates Incubate Serum Dilution Human RSV Reference Serum
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