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Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry

Article DOI: 10.3791/59028-v • 08:24 min • March 14th, 2020
March 14th, 2020 •
1, 1, 1
1Biomedical Innovation Department, Center for Scientific Research and Higher Education at Ensenada

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The ex vivo culture of bone explants can be a valuable tool for the study of bone physiology and the potential evaluation of drugs in bone remodeling and bone diseases. The presented protocol describes the preparation and culture of calvarias isolated from newborn mice skulls, as well as its applications.

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Bone Remodeling Tumor-bone Microenvironment Calvaria Co-culture Histomorphometry Ex Vivo Model Cancer Cells Therapeutic Potential Diverse Molecules Bone-tumor Microenvironment Mouse Calvarial Organ Culture Bone Regeneration Mouse Pup Sutures Of The Skull Sagittal Coronal Lambdoids Dissect Calvaria Scalp Area Anterior Fontanel Tissue Inclusion
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