7,361 Views
•
11:11 min
February 21, 2019
DOI:
10.3791/59037-v
该协议的目的是标记、丰富和识别复杂生物样品 (如细胞裂解物或组织均质) 中的蛋白激酶 ck2 底物。此方法利用 ck2 生物学的独特方面来实现这一目的。
06:51
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Related Videos
12958 Views
08:59
Defining Substrate Specificities for Lipase and Phospholipase Candidates
14908 Views
08:05
Assaying Protein Kinase Activity with Radiolabeled ATP
18337 Views
12:26
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
18587 Views
07:18
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
6438 Views
11:23
Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
6190 Views
13:22
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
7863 Views
06:09
Assaying the Kinase Activity of LRRK2 in vitro
22529 Views
08:07
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
8007 Views
08:48
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
13922 Views
Read Article
Cite this Article
Chojnowski, J. E., McMillan, E. A., Strochlic, T. I. Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach. J. Vis. Exp. (144), e59037, doi:10.3791/59037 (2019).
Download .ris file
Copy
Share Video
.