rad51 介导的 dna 链交换反应的实时观测
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06:24 min
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February 13, 2019
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Summary
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建立了以光谱51介导的 dna 链交换反应为基础的荧光共振能量转移实时观测系统。利用这里提出的协议, 我们能够检测反应中间体的形成及其转化为产物, 同时也分析了反应的酶动力学。
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Cite this Article
Ito, K., Argunhan, B., Tsubouchi, H., Iwasaki, H. Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51. J. Vis. Exp. (144), e59073, doi:10.3791/59073 (2019).
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