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JoVE Journal
Behavior
斑马鱼内生应激研究的行为方法
斑马鱼内生应激研究的行为方法
JoVE Journal
Behavior
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JoVE Journal Behavior
Behavioral Approaches to Studying Innate Stress in Zebrafish

斑马鱼内生应激研究的行为方法

Full Text
9,733 Views
05:29 min
May 1, 2019

DOI: 10.3791/59092-v

Jacqueline S.R. Chin1, Lydia T. Albert2, Cody L. Loomis1,3, Alex C. Keene1,3, Erik R. Duboué1,2,3

1Jupiter Life Science Initiative,Florida Atlantic University, 2Wilkes Honors College and Florida Atlantic University, 3Charles E. Schmidt College of Science,Florida Atlantic University

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Please note that some of the translations on this page are AI generated. Click here for the English version.

这份手稿描述了一种测量成年斑马鱼压力行为的简单方法。该方法利用了斑马鱼在压力状态下更喜欢鱼缸的下半部分的固有倾向。我们还描述了将检测与药理学结合的方法。

新的坦克检测将结合强大的基因工具在斑马鱼提供了一种方法,解剖大脑如何调节压力和神经回路如何可能解除调节。斑马鱼和哺乳动物之间的遗传和神经元信号通路高度保守,这种技术可以帮助我们进一步理解压力反应背后的机制。该方法不是斑马鱼特有的,已经用于墨西哥盲洞鱼,因此有可能扩展到其他鱼类物种。

试运行可能有助于确定动物与背景的正确照明和对比度,以实现成功的跟踪。检测很少使用,但可以是一个涉及的过程。可视化这些步骤将有助于其他研究人员成功执行该过程。

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