一种在裂变酵母中的深度固序辅助自发抑制屏
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07:55 min
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March 07, 2019
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Summary
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我们提出了一个简单的抑制屏幕协议在裂变酵母。这种方法是有效的, 无突变, 并选择性的突变, 经常发生在一个单一的基因组位点。 该协议适用于分离抑制剂, 以减轻由突变或药物引起的液体培养中的生长缺陷。
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Cite this Article
Marayati, B. F., Pease, J. B., Zhang, K. A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe. J. Vis. Exp. (145), e59133, doi:10.3791/59133 (2019).
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