Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Sidney Mahan; Mingjun Liu; Richard  A. Baylis; Delphine Gomez

doi:10.3791/59139

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Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

平滑肌细胞近系追踪小鼠晚期动脉粥样硬化病变细胞成分的定量分析

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09:06 min

February 20, 2019

DOI: 10.3791/59139-v

Sidney Mahan¹, Mingjun Liu¹, Richard A. Baylis^2,3, Delphine Gomez^1,4

¹Heart, Lung, Blood and Vascular Medicine Institute,University of Pittsburgh, ²Robert M. Berne Cardiovascular Research Center,University of Virginia, ³Department of Biochemistry and Molecular Genetics,University of Virginia, ⁴Division of Cardiology,University of Pittsburgh School of Medicine

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我们提出了一个标准化的方案来表征晚期小鼠动脉粥样硬化病变的细胞组成, 包括动物解剖、组织包埋、切片、染色和腕头动脉分析的系统方法从动脉粥样硬化平滑肌细胞谱系追踪小鼠。

我们的协议帮助研究人员实施标准化的实验管道，通过免疫荧光染色对类似人群进行表型表征。通过这项技术，研究人员可以严格分析复杂疾病组织的类似组成，对感兴趣的细胞类型的表型进行定性和定量分析。演示这个程序的将是西德尼·马汉，一个来自我实验室的技术人员。

为使动物为胸腺动脉的收获做好准备，将腹膜切到胸骨，注意不要损害腹部组织。并在中轴线通过胸部进行两次切口，注意不要损害心脏和肺部。然后，切开隔膜，部分暴露心脏。

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医学第144期动脉粥样硬化动脉粥样硬化病变臂头动脉组织准备和切片免疫组织化学免疫荧光共聚焦显微镜