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Bioengineering

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Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
 

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

Article DOI: 10.3791/59173-v 09:57 min April 1st, 2019
April 1st, 2019

章节

总结概括

Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.

Tags

Keywords: Tissue-engineered Tracheal Graft Mouse Model Orthotopic Tracheal Replacement Airway Regeneration Scaffold Electrospinning Polyethylene Terephthalate (PET) Polyurethane (PU) Microsurgical Techniques Anesthesia Graft Handling Suture Placement
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