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In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
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In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

Article DOI: 10.3791/59286-v • 10:54 min • June 7th, 2019
June 7th, 2019 •
1, 1, 1, 1, 1, 1, 1,2
1Department of Neuroscience, Faculty of Medicine and Health Science, University of Copenhagen, 2Department of Clinical Neurophysiology, Rigshospitalet

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We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.

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In Vivo Three-Dimensional Two-Photon Microscopy Conducted Vascular Responses Local ATP Ejection Glass Micro-Pipette Slow Conducted Vascular Responses Cerebral Capillaries Local Synaptic Activities 3D Two-photon Microscopy Cerebral Neuroma Glial Activity Brain Function Oxygen And Nutrition Supply Complex Network Of Vessels Cerebral Blood Flow (CBF) Capillary Level In Vivo Two-photon Microscopy Studies CBF Responses Localized Interventions Vascular Network Hyperstack Two-photon Imaging
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