混合光/蓝色原生电泳在线粒体呼吸链超综合体分离与分析中的作用
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11:25 min
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May 19, 2019
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在这里, 我们提出了一个协议, 以提取, 解决和识别线粒体超级复合物, 最大限度地减少接触洗涤剂和库马西蓝。该协议提供了分辨率和酶活性保存之间的最佳平衡, 同时最大限度地降低了失去不稳定的蛋白质-蛋白质相互作用的风险。
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Cite this Article
Cuillerier, A., Burelle, Y. Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes. J. Vis. Exp. (147), e59294, doi:10.3791/59294 (2019).
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