Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Zhiping Zhang; Shuhang Fan; Zhiqiang Chen; Yanzhi He; Mengfei Huang; Li Ding; Yanyan Zhang; Lin Chen; Xinyue Zhang

doi:10.3791/59336

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

通过建立单罐双酶级联,从弗拉瓦内生物合成一种黄酮

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09:50 min

August 14, 2019

DOI: 10.3791/59336-v

Zhiping Zhang¹, Shuhang Fan¹, Zhiqiang Chen¹, Yanzhi He¹, Mengfei Huang¹, Li Ding¹, Yanyan Zhang⁵, Lin Chen¹, Xinyue Zhang^1,2,3,4

¹College of Bioscience and Biotechnology,Yangzhou University, ²Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China,Yangzhou University, ³Joint International Research Laboratory of Agriculture & Agri-Product Safety,Yangzhou University, ⁴Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, ⁵The Testing Center,Yangzhou University

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黄酮醇的衍生对于其在医疗保健和食品工业中的应用至关重要。在这里,我们为黄酮中黄酮的生物合成提供详细的方案,并讨论与其他方法不同的关键步骤及其优势。

使用该协议，我们可以轻松地在一个锅中从黄酮中产生大量的黄酮。这种技术省时、省、省、地高、易于控制，具有巨大的产业化潜力。是的，此方法可以提供其他二级代谢物的经济生产见解，但应用到它时需要一些修改。

他通常一无所获或产量很低。我的建议是处理冰上重组酶，并在酶的库存溶液中加入10%甘油。这是因为只有通过视觉演示，新手才能了解影响实验成功实施的重要细节和真相。

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生物化学问题 150 黄酮黄酮卡温弗罗醇奎西汀生物合成多酶双酶黄烷3羟基酶黄酮合成酶