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Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
 

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Article DOI: 10.3791/59428-v 08:10 min May 2nd, 2019
May 2nd, 2019

章节

总结概括

A pan-lyssavirus nested reverse transcription polymerase chain reaction has been developed to detect specifically all known lyssaviruses. Validation using rabies brain samples of different animal species showed that this method has a sensitivity and specificity equivalent to the gold standard fluorescent antibody test and could be used for routine rabies diagnosis.

Tags

Nested RT-PCR Lyssaviruses Universal Detection Clinical Rabies Diagnosis Surveillance PCR Carry-over Contamination Negative Control Positive Control RNA Extraction Reverse Transcription Rabies Contamination Peptide Chain Ribonuclease Free Materials
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