Assessing the Cellular Immune Response of the Fruit Fly, <em>Drosophila melanogaster</em>, Using an In Vivo Phagocytosis Assay

Ashley E. Nazario-Toole; Louisa  P. Wu

doi:10.3791/59543

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

果蝇、果蝇细胞免疫应答的评估

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05:50 min

April 10, 2019

DOI: 10.3791/59543-v

Ashley E. Nazario-Toole^1,2, Louisa P. Wu^2,3

¹Department of Biology,University of Maryland, ²Department of Cell Biology and Molecular Genetics,University of Maryland, ³Institute for Bioscience and Biotechnology Research,University of Maryland

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该方案描述了一种在成虫体内吞噬试验的方法, 以量化吞噬细胞的识别和微生物感染的清除。

这种体内吞噬分析使研究人员能够进行基因筛选和全基因组关联研究，以确定调节成人血细胞中吞噬的新基因。该实验是定量的，易于执行，可用于活的动物的宿主因素的筛选，影响病原体识别、吸收和清除。用拔针器拉薄壁玻璃毛细管后，使用千分尺将针头放在显微镜下，并使用五号细点不锈钢钳子将尖端断裂到100微米的尖端直径。

要测量将注入到每只苍蝇中的液体体积，在 PBS 中装载带无菌 5% 食品色素的毛细管针，并将液体排出到 0.01 毫米级微米的矿物油滴上。将每毫升颗粒10微升1.6毫克的颗粒分配到小方的 Parafilm 上，然后将液体拉入针头。将针头安装到喷油器喷嘴中，沿着飞垫上指定的区域将麻醉苍蝇线，腹侧朝上，头部朝向喷油器前部。

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