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被引用 3 次
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13:27 分钟
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2019年8月8日
2019年8月8日
•本方案描述了使用声波液滴喷射技术在384孔板中对哺乳动物细胞进行高通量质粒转染的方法。耗时且易出错的DNA分配与多重化操作,以及转染试剂的分配,均由软件驱动并通过纳升级分液设备完成。随后将细胞接种至这些预先加样好的孔中。
Chapters in this video
0:04
Title
0:47
Preparation of the Peristaltic Liquid Handler Programs
1:53
Experimental Design and Generation of the Picklists to Drive the Acoustic Droplet Ejection (ADE)-Based Dispenses
3:34
DNA Source Plate Preparation Using the 384-well Pipetting Guide Application and Survey Performance to Control the Manually Dispensed Volumes
6:18
Peristaltic Liquid Handler-Based 1 μL Diluent Dispensation in the Destination Plate
7:03
ADE-driven DNA Dispensing into the Destination Plate and ADE-driven Transfection Reagent Dispensing
9:54
Peristaltic Liquid Handler-Based Cell Dispensation
10:51
Results: Defining the Key Parameters to Reach Highly Efficient and High Thoughput Cells (Co-) Transfections
12:26
Conclusion
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