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Isolation of Atrial Myocytes from Adult Mice
JoVE 杂志
医学
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JoVE 杂志 医学
Isolation of Atrial Myocytes from Adult Mice

Isolation of Atrial Myocytes from Adult Mice

DOI:
10.3791/59588-v

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08:34 min

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July 25, 2019

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1Libin Cardiovascular Institute of Alberta, Department of Cardiac Sciences, Department of Physiology and Pharmacology, Cumming School of Medicine,University of Calgary

Chapters

  • 00:04Title
  • 00:42Dissection of Mouse Atrial Appendage(s)
  • 02:46Isolation of Atrial Myocytes
  • 06:19Results: Representative Patch-clamp Data Obtained From Isolated Mouse Atrial Myocytes
  • 07:52Conclusion

Summary

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自动翻译

This protocol is used to isolate single atrial cardiomyocytes from the adult mouse heart using a chunk digestion approach. This approach is used to isolate right or left atrial myocytes that can be used to characterize atrial myocyte electrophysiology in patch-clamp studies.

Tags

Atrial MyocytesPatch-clampAtrial ElectrophysiologyArrhythmogenesisTrunk DigestionMouse EuthanasiaAtrial AppendageModified Tyrode’s Solution

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