富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定
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10:21 min
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June 20, 2019
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Summary
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用实例描述了含紫酯水酶的表达和纯化(在大肠杆菌、FPLC中的表达)。纯化蛋白用于结晶和抗体生产,并用于酶测定。提出了选定的光度测定法,以显示FAHD1的多功能性,如牛酸脱碳酶和丙烯酰胺水酶。
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Cite this Article
Weiss, A. K. H., Holzknecht, M., Cappuccio, E., Dorigatti, I., Kreidl, K., Naschberger, A., Rupp, B., Gstach, H., Jansen-Dürr, P. Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins. J. Vis. Exp. (148), e59729, doi:10.3791/59729 (2019).
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