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JoVE Journal Genetics
Poolade CRISPR-baserade genetiska skärmar i däggdjursceller
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Journal (Genetics)
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
Journal (Genetics)
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
 
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Poolade CRISPR-baserade genetiska skärmar i däggdjursceller

Article DOI: 10.3791/59780-v • 09:05 min • September 4th, 2019
September 4th, 2019 •
*1, *1, 1, 1, 1,2,3
1Donnelly Centre, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto
* These authors contributed equally

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CRISPR-Cas9 teknik ger en effektiv metod för att exakt redigera däggdjur arvsmassan i någon celltyp och representerar ett nytt sätt att utföra genomomfattande genetiska skärmar. Ett detaljerat protokoll som diskuterar de steg som krävs för lyckad prestanda av poolade genomhela CRISPR-Cas9 skärmar finns här.

Tags

Genetik CRISPR-Cas9 sgRNA bibliotek Toronto-knock-out genomomfattande CRISPR screening poolade Drop-out skärmar funktionsgenomik viktiga gener cell Fitness spridning
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