13,432 Views
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08:21 min
September 01, 2019
DOI:
10.3791/59968-v
该方法结合了DNA双链断裂(DSB)、细胞循环分布和凋亡的定量分析,实现了对DSB诱导和修复以及修复失败后果的细胞周期特定评估。
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Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
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Cite this Article
Lopez Perez, R., Münz, F., Kroschke, J., Brauer, J., Nicolay, N. H., Huber, P. E. Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry. J. Vis. Exp. (151), e59968, doi:10.3791/59968 (2019).
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